What is the role of biological buffer PEP in the reagent kit?

Product Details

1、 Introduction to PEP buffer solution

PEP buffer, also known as phosphoenolpyruvate tris (cyclohexylamine) salt, CAS number 35556-70-8, with a purity of over 99%. It is a very important chemical substance in life activities and can participate in various biochemical processes. At the same time, whether in the animal or plant kingdom, it can participate in cellular respiration and photosynthesis processes.

2、 PEP Application in Serum Carbon Dioxide Determination Kit

Due to the ability of PEP to perform photosynthesis, CO2 can be absorbed and converted into oxaloacetic acid through the catalytic action of PEP carboxylase throughout the entire process. Due to its high efficiency in fixing carbon dioxide and highly sensitive reaction, this characteristic allows for the determination of trace amounts of CO2 in serum, as well as the determination of PEP carboxylase activity in plant samples or plasma.

3、 Application of PEP in Cell Protectants and Antioxidants

During the process of cellular respiration, it can participate in glycolysis reactions, removing high-energy phosphate groups and converting them into pyruvate. This can reduce oxidative stress and ATP consumption of tissue cells during organ tissue refrigeration, reduce organ damage, and extend clinical organ preservation time.

As a professional manufacturer of biological buffering agents, Desheng produces a wide range of products including Tris, Caps, MOPS, PEP, etc. It can provide customers with one-stop procurement. Currently, the PEP solution sold is mainly used in carbon dioxide measurement and PEP carboxylase activity measurement kits, which are highly popular in the market. Recently, there has been a gradual increase in the number of people purchasing PEP. Desheng provides customized services based on customer needs, bringing welfare benefits to more customers. If you have any related needs, please click on the website or call to inquire about the details!