Application Of N - (2-Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid (EPPS) Buffer

Product Details

EPPS, also known as N - (2-hydroxyethyl) piperazin-N '-3-propanesulfonic acid, is an important buffering agent with a CAS of 16052-06-5 and a molecular weight of 252.33. Due to its excellent buffering performance, it is widely used in fields such as biochemistry, molecular biology, and biomedical research, making it an indispensable product in the laboratory. So what are its uses?

1、 DNA and RNA Studies

EPPS buffer is commonly used in DNA and RNA screening systems. DNA and RNA research is one of the core research areas in biology and molecular biology, and the use of EPPS enables researchers to conduct experiments under specific pH conditions, ensuring the accuracy and repeatability of experimental results.

2、 Chloroplast Studies

EPPS buffer has a wide range of applications in the study of electron transfer and phosphorylation of chloroplast thin layer preparation. Chloroplasts are key organelles for photosynthesis in plant cells. Studying the function and biochemical processes of chloroplasts is crucial for understanding plant growth and development. EPPS buffer can provide a stable chemical environment, which helps to maintain the smooth progress of experiments.

3、 Oxyhemoglobin protection

Due to the potential interference of methemoglobin with experimental results, EPPS buffer is very useful in preparing protein samples and can be used to protect oxygenated hemoglobin from oxidation to methemoglobin during freeze-drying.

4、 Capillary zone electrophoresis

EPPS can also be used as a background electrolyte for capillary zone electrophoresis for protein microanalysis. Capillary zone electrophoresis is an efficient analytical technique widely used in biochemical and molecular biology research. As a background electrolyte, EPPS helps to maintain the stability and repeatability of the analysis.

From the above applications, EPPS buffer has extensive applications in fields such as DNA and RNA research, chloroplast research, protection of oxygenated hemoglobin, and capillary zone electrophoresis. Its superior performance makes it a popular product in the market, and it is important to carefully consider manufacturers with stable supply when choosing.

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