PAPANICOLAOU STAIN/ RAPID PAP STAIN KIT FOR SALE

Product Details

PAPANICOLAOU STAIN

Intended use:

Study of morphology of squamous epithelial cell

Principle:

Papanicolau Stains are used in conjunction with Hematoxylin nuclear stains in the diagnosis of malignant cytological disease. EA-36 and EA-50 are used in conjunction with OG-6 for gynecological staining. EA-65 is used with OG-6 for non-gynecological staining. When performed properly, the stained specimen should display hues from the entire spectrum: red, orange, yellow, green, blue, and violet. The chromatin patterns are well visible, the cells from borderline lesions are easier to interpret and the photomicrographs are better. The staining results in very transparent cells, so even thicker specimens with overlapping cells can be interpreted.

Kit components:

Reagent bottles

Kit insert.

Physical Appearance:

Hematoxylin clear wine red coloured liquid without any particles.

OG-6 clear orange color liquid without any particles.

Light green SF green color clear liquid with reddish purple tinge without any particles.

Dehydrant colorless liquid without any particles.

Xylene colorless liquid without any particles.

Ana fix colorless liquid without any particles.

Pack Size:

250 SMEARS

PROCEDURE:

1. Prepare a smear on clear, dry glass slide.

2. Fix with Ana fix.

3. Pour Dehydrant Reagent on slide for 3 minutes.

4. Wash with distilled water.

5. Flood the smear with Hematoxylin reagent for 1 min.

6. Add 3 drops of Phosphate Buffer which is not provided for 10 seconds.

7. Wash with Dehydrant 2 times for 30 seconds.

8. Pour Working Stain on slide. Allow it to remain for 45 seconds.

9. Wash with Dehydrant for 30 seconds.

10. Rinse with Xylene.

11. Allow the slide to air dry.

12. Mount with glass cover using a drop of DPX.