Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit（96tests/box）

The difficulty of cold chain transportation of Novel Coronavirus nucleic acid detection reagent
When conventional nucleic acid detection reagents are transported in long distance, the (-20±5) ℃ cold chain storage and transportation is required to ensure the bioactive of enzyme in the reagents remain active. To make sure that the temperature reaches the standard, several kilograms of dry ice are needed for each box of nucleic acid testing reagent even less than 50g , but it can only last for two or three days. On the perspective of industry practice, the actual weight of reagents issued by manufacturers is less than 10% (or far less than this value) of the container. Most of the weight comes from dry ice, ice packs and foam boxes, so the transportation cost is extremely high.

In March 2020, COVID-19 began to break out in a large scale abroad, and the demand for Novel Coronavirus nucleic acid detection reagent increased dramatically. Despite the high cost of exporting the reagents in the cold chain, most manufacturers can still accept it due to the large quantity and high profit.

However, with the improvement of national export policies for anti-pandemic products, as well as the upgrading of national control over the flow of people and logistics, there is extension and uncertainty in the transportation time of reagents, which resulted in prominent product problems caused by the transportation. Extended transportation time (transportation time of about half a month is very common) leads to frequent product failures when the product reaches the client. This has troubled most nucleic acid reagents export enterprises.

Lyophilized technology for PCR reagent helped the transportation of Novel Coronavirus nucleic acid detection reagent worldwide

The lyophilized PCR reagents can be transported and stored at room temperature, which can not only reduce the transportation cost, but also avoid the quality problems caused by transportation process. Therefore, lyophilizing the reagent is the best way to solve the problem of export transportation.

Lyophilization involves freezing a solution into a solid state, and then sublimate and separate the water vapor under vacuum condition. The dried solute remains in the container with the same composition and activity. Compared with conventional liquid reagents, the full-component lyophilized Novel Coronavirus nucleic acid detection reagent produced by Liming Bio has the following characteristics:

极强的热稳定性： 可在56℃下静置处理60天，试剂的形态和性能保持不变。
常温储运： 无需冷链，开封前无需低温储存，充分释放冷库空间。
即用型： 所有组分冻干，无需系统配置，避免酶等高粘度组分流失。
一管多重靶标：检测靶标涵盖新型冠状病毒ORF1ab基因、N基因、S基因，避免病毒基因变异。为减少假阴性，采用人RNase P基因作为内参，以满足临床对样品质量控制的需要。