TRIS HYDROCHLORIDE CAS NO.1185-53-1

Product Details

Tris hydrochloride raw material, or tri(hydroxymethyl)aminomethane hydrochloride, is a very common biological buffer, widely used in nucleic acids, protein-related experiments buffer, protein, nucleic acid electrophoresis solution, cell and other microbial culture solutions, virus preservation solutions and so on.

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Tris Hydrochloride Buffer Details

Tris HCl powder is most commonly used Tris buffer, in addition to acetate CH3COOH, boric acid, phosphoric acid, glycerine, malic acid, EDTA, etc., as buffer, electrophoresis solution and so on. The ionic strength of Tris hydrochloride buffer is very low, while other buffers need to add NAOH alkali, and the ionic strength is high. Tris hydrochloride pH 7.5 to 8.5.

Tris Hydrochloride Buffer Product Advantages:

Tris hydrochloride is a solvent for nucleic acids and proteins;

Tris HCl powder has good water solubility, good buffer performance, and large buffer capacity;

White crystal powder, which can be sealed and stored for a long time;

It does not form a precipitate with calcium, magnesium and other metal ions.

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1185-53-1 Tris HCl Use

Tris HCl powder is used for protein crystal growth under different pH conditions. Low ionic strength characteristics can be used for nematodes (C. elegans). elegans) formation of intermediate fibers of the nuclear fibrinolin (lamin). Tris HCl buffer is also one of the main components of protein electrophoresis buffer, which forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris hydrochloride buffer is an intermediate in the preparation of surfactants, vulcanization promoters and some drugs. Tris is also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tric HCl powder is not suitable and it is best to switch to a buffer that can be tolerated by other mass spectrometers.

Tris Hydrochloride Raw Material CAS NO. 1185-53-1 Instructions:

Tris hydrochloride raw material can be used directly in solution water, according to the desired concentration, a certain amount of Tris hydrochloride powder can be dissolved in distilled water, and buffers with different pH values can also be obtained by different ratios of Tris to hydrochloric acid.

Tris Hydrochloride CAS NO. 1185-53-1 precautions

The pH of Tris hydrochloride buffer powder is greatly affected by the solution concentration, the buffer is diluted tenfold, and the pH value changes greater than 0.1;

The temperature effect is large, and the temperature change has a great influence on the pH value of this kind of biological buffer;

The tris hydrochloride raw material is easy to absorb CO2 in the air, so it should be tightly sealed;

This tris hydrochloride buffer has a certain interference effect on some pH electrodes, so use electrodes that are compatible with Tris solutions. The configured tris hydrochloride solution is recommended to be used now.

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