SL0005Hu Human Fatty Acid Binding Protein 4(FABP4) ELISA Kit (SL0005Hu)

Our Human Fatty Acid Binding Protein 4(FABP4)ELISA kit is to assay FABP4 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FABP4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for FABP4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FABP4 and HRP conjugated FABP4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FABP4. You can calculate the concentration of FABP4 in the samples by comparing the OD of the samples to the standard curve.

Advantages of these ELISA kits:

• Thousands of kits provided
• Optimized for sensitive, accurate, and consistent performance
• Thorough instructions to complete protocol in 2.5 to 4 hours (varies by kit)
• Validated for typical sample types (e.g., serum, plasma, supernatant, lysates)

Details of the products picture

Materials provided with the kit