NeoPlex™ STI-7 PLUS Detection Kit

Product Details

[INTENDED USE]

The 'NeoPlexTM STI-7 PLUS Detection kit' is a qualitative in vitro test for the simultaneous detection and confirmation

of seven (7) sexually-transmitted infection (STI)-causing pathogens including Candida albicans (CA), Gardnerella

vaginalis (GV), Treponema pallidum (TP), Haemophilus ducreyi (HD), Herpes simplex virus -1 (HSV-1), Herpes

simplex virus -2 (HSV-2) and Streptococcus agalactiae (GBS) from urine or vaginal swab specimens.

[PRINCIPLE OF ASSAY]

'NeoPlexTM STI-7 PLUS Detection kit' is based on two major processes, isolation of DNA from specimens and

multiplex real-time amplification. STI-causing pathogens DNA is extracted from the specimens, amplified in multiplex

real-time PCR and detected using fluorescent reporter dye probes specific for STIs-causing pathogens DNA and

Internal Control. Internal Control (IC) serves as an amplification control for each individually processed specimen and

to identify possible reaction inhibition.

[Additional required equipment and materials]

-CFX96 real-time PCR detection system (BioRad, Inc., Cat No. 1845097-IVD) or equivalent

-0.2 ml 8-Tube PCR Strips without Caps, low profile, white (BioRad, Inc., Cat No. TLS0851)

-Optical Flat 8-Cap Strips for PCR Tubes (BioRad, Inc., Cat No. TCS0803)

-Multiplate™ 96-Well PCR Plates, low profile, unskirted, white (BioRad, Inc., Cat No. MLL9651)

-QIAamp DSP DNA Mini Kit (QIAGEN, Cat No.61304) or equivalent DNA extraction kit

-Pipettes set

-Micro Centrifuge