Human PD-1/PDCD1(Programmed Cell Death Protein 1) ELISA Kit

Product Details

Human PD-1/PDCD1(Programmed Cell Death Protein 1) ELISA Kit Basic Information

Feiyue’s Human PD-1/PDCD1 is an ELISA reagent for detection of PD-1/PDCD1 in serum, plasma or cell with sensitivity, specificity and consistency.

Human PD-1/PDCD1(Programmed Cell Death Protein 1) Introduciton

Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).

Human PD-1/PDCD1(Programmed Cell Death Protein 1) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human PD-1/PDCD1.

Human PD-1/PDCD1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human PD-1/PDCD1 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human PD-1/PDCD1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.