This kit is established with a colloidal gold immunochromatography technique for the rapid detection of IgM and IgG antibodies against 2019-nCoV in serum, plasma or whole blood samples. The colloidal gold labeled recombinant Np Ag and mouse IgG colloidal gold marker are immobilized on the conjugate pad, mouse anti human IgM monoclonal antibody (μ) and mouse anti human IgG monoclonal antibody (γ) was pre-coated on the detection line (T) position on the nitrocellulose membrane respectively. The control line(C) was coated with goat anti-mouse IgG polyclonal antibody. When an appropriate amount of samples to be tested is added to the sample well of the test cassette, the sample will move forward by chromatography. If the sample contains antibodies against the 2019-nCoV, the antibodies can combine with colloidal gold labeled recombinant Np Ag to form an immune complex. The immune complex will flow forward undergoing the inside of the nitrocellulose membrane, and will be caught by the coated mouse anti human IgM antibody (μ)or anti human IgG antibody (γ)to develop color when it passed the T line position. The free colloidal gold marker will be caught by the goat anti-mouse IgG polyclonal antibody coated on the C line to develop color. Negative samples develop color only at the C line.
18 Industrial Development Zone Keyuan Road, Huangcun Town, Daxing District, Beijing