BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit

Product Details

E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit

Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samples from Canine species. β Cell Leukemia/Lymphoma 2 can also be called BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL 2, B-cell leukemia/lymphoma 2, Bcl-2 alpha, Apoptosis regulator Bcl-2, Bcl-2-like protein 1, Bcl-2-associated X protein, Bcl-2 homologous antagonist/killer, Bcl-2-related ovarian killer protein, Bcl-w, Bcl-xL, Bcl-xS, Protein phosphatase 1 regulatory subunit 52, PPP1R5.

Specifications of Canine β Cell Leukemia/Lymphoma 2 ELISA kit

Product Information
Cat. No.	E08B0033
Product Name	Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	10-25 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

BCL2 ELISA applies the competitive enzyme immunoassay technique utilizing an anti-BCL 2 antibody and an BCL 2-HRP conjugate. The assay sample and buffer are incubated together with BCL 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL 2 concentration since BCL 2 from samples and BCL 2-HRP conjugate compete for the anti-BCL 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL 2 from the sample, fewer sites are left to bind BCL 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL 2 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine β Cell Leukemia/Lymphoma 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	86-107%
Linearity	Diluent Ratio	Range %
	1:02	86-109
	1:04	83-111
	1:08	83-115
Specificity/ Cross-reactivity	No significant cross-reactivity or interference between BCL 2 and analogues was observed.

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