BlueGene Biotech Canine Ferritin ELISA kit

Product Details

E08F0010 Canine Ferritin ELISA kit

Canine Ferritin ELISA kit is suitable for the detection of samples from Canine species. Ferritin can also be called FE.

Specifications of Canine Ferritin ELISA Test kit

Product Information
Cat. No.	E08F0010
Product Name	Canine Ferritin ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	25-500 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	25 ng/mL	1 vial
STANDARD C (0.5mL)	50 ng/mL	1 vial
STANDARD D (0.5mL)	100 ng/mL	1 vial
STANDARD E (0.5mL)	250 ng/mL	1 vial
STANDARD F (0.5mL)	500 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

FE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FE antibody and an FE-HRP conjugate. The assay sample and buffer are incubated together with FE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FE concentration since FE from samples and FE-HRP conjugate compete for the anti-FE antibody binding site. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Ferritin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	92-108%
Linearity	Diluent Ratio	Range %
	1:02	87-113
	1:04	82-116
	1:08	84-115
Specificity/Cross-reactivity	No significant cross-reactivity or interference between FE and analogues was observed.

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