BlueGene Biotech Bovine Catalase ELISA kit

Product Details

E11C0086 Bovine Catalase ELISA kit

Bovine Catalase ELISA kit is suitable for the detection of samples from Bovine species. Catalase can also be called Cas1, CAT, Cs1.

Specifications of Bovine Catalase ELISA kit

Product Information
Cat. No.	E11C0086
Product Name	Bovine Catalase ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	10-250 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	10 ng/mL	1 vial
STANDARD C (0.5mL)	25 ng/mL	1 vial
STANDARD D (0.5mL)	50 ng/mL	1 vial
STANDARD E (0.5mL)	100 ng/mL	1 vial
STANDARD F (0.5mL)	250 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Catalase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-105%
Linearity	Diluent Ratio	Range %
	1:2	87-108
	1:4	89-109
	1:8	85-111
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CAT and analogues was observed.

As one of mouse igg elisa kits manufacturers, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGene Biotech ELISA kits.