BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit

Product Details

E11C0339 Bovine C Type Natriuretic Peptide ELISA kit

Bovine C Type Natriuretic Peptide ELISA kit is suitable for the detection of samples from Bovine species. C Type Natriuretic Peptide can also be called NPPC; CNP2; Natriuretic peptide precursor C; natriuretic peptide C, CTNP.

Specifications of Bovine C Type Natriuretic Peptide ELISA kit

Product Information
Cat. No.	E11C0339
Product Name	Bovine C Type Natriuretic Peptide ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP peptide concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine C Type Natriuretic Peptide ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	90-101%
Linearity	Diluent Ratio	Range %
	1:2	87-105
	1:4	84-108
	1:8	81-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CNP and analogues was observed.

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