Bovine Carnitine Palmitoyltransferase I ELISA kit
Metabolism
E11C0055 Bovine Carnitine Palmitoyltransferase I ELISA kit
Bovine Carnitine Palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyltransferase i (CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.
Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit
Product Information |
|
Cat. No. |
E11C0055 |
Product Name |
Bovine Carnitine palmitoyltransferase I ELISA kit |
Species |
Bovine |
Product Size |
48 Tests / 96 Tests |
Concentration |
2.5-50 ng/mL |
Sensitivity |
0.1 ng/mL |
Principal |
Competitive ELISA |
Sample Volume |
100 ul |
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time |
90 minutes |
Platform |
Microplate Reader |
Conjugate |
HRP |
Detection Method |
Colorimetric |
Storage |
2-8°C |
Kit Components |
||
MATERIALS |
SPECIFICATION |
QUANTITY |
MICROTITER PLATE |
96 wells |
stripwell |
ENZYME CONJUGATE |
6.0 mL |
1 vial |
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
STANDARD B (0.5mL) |
2.5 ng/mL |
1 vial |
STANDARD C (0.5mL) |
5 ng/mL |
1 vial |
STANDARD D (0.5mL) |
10 ng/mL |
1 vial |
STANDARD E (0.5mL) |
25 ng/mL |
1 vial |
STANDARD F (0.5mL) |
50 ng/mL |
1 vial |
SUBSTRATE A |
6 mL |
1 vial |
SUBSTRATE B |
6 mL |
1 vial |
STOP SOLUTION |
6 mL |
1 vial |
WASH SOLUTION (100 x) |
10 mL |
1 vial |
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay |
The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.
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